The inhibition kinetics of yeast glutathione reductase by some metal ions.
作者： Berivan Tandoğan , N. Nuray Ulusu
DOI: 10.1080/14756360601162147
关键词: Yeast 、 Zinc 、 Inorganic chemistry 、 Glutathione 、 Chelation 、 Calcium 、 Cadmium 、 Glutathione reductase 、 Chemistry 、 Nuclear chemistry 、 Metal ions in aqueous solution
摘要: Glutathione reductase (GR, type IV, Baker's yeast, E.C 1.6.4.2) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced (GSH). In this study some metal ions have been tested on GR; lithium, manganese, molybdate, aluminium, barium, zinc, calcium, cadmium and nickel. Cadmium, nickel calcium showed good moderate inhibitory effect yeast GR. GR inhibited non-competitively by Zn2 + (up 2 mM) activated above concentration. Ca2 inhibition was non-competitive with respect GSSG uncompetitive NADPH. Nickel competitive The constants for these metals were determined. chelating agent EDTA recovered 90% activity metals.
informapharmascience.com参考文章(38)
Vincent Massey, Charles H. Williams, On the reaction mechanism of yeast glutathione reductase. Journal of Biological Chemistry. ,vol. 240, pp. 4470- 4480 ,(1965) , 10.1016/S0021-9258(18)97085-7
James P. McClung, Angus G. Scrimgeour, Zinc: An Essential Trace Element with Potential Benefits to Soldiers Military Medicine. ,vol. 170, pp. 1048- 1052 ,(2005) , 10.7205/MILMED.170.12.1048
Klaus Hantke, Bacterial zinc transporters and regulators. Biometals. ,vol. 14, pp. 239- 249 ,(2001) , 10.1023/A:1012984713391
Elizabeth Somer, The Essential Guide to Vitamins and Minerals ,(1991)
I Carlberg, B Mannervik, Purification and characterization of the flavoenzyme glutathione reductase from rat liver. Journal of Biological Chemistry. ,vol. 250, pp. 5475- 5480 ,(1975) , 10.1016/S0021-9258(19)41206-4
A Serrano, J Rivas, M Losada, Purification and properties of glutathione reductase from the cyanobacterium Anabaena sp. strain 7119. Journal of Bacteriology. ,vol. 158, pp. 317- 324 ,(1984) , 10.1128/JB.158.1.317-324.1984
A Bashir, R N Perham, N S Scrutton, A Berry, Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase Biochemical Journal. ,vol. 312, pp. 527- 533 ,(1995) , 10.1042/BJ3120527
Christian T. Sheline, M. Margarita Behrens, Dennis W. Choi, Zinc-induced cortical neuronal death: contribution of energy failure attributable to loss of NAD(+) and inhibition of glycolysis. The Journal of Neuroscience. ,vol. 20, pp. 3139- 3146 ,(2000) , 10.1523/JNEUROSCI.20-09-03139.2000
U.I. Walther, B. Wilhelm, S.C. Walther, H. Mückter, W. Forth, Effect of zinc chloride on GSH synthesis rates in various lung cell lines. in Vitro & Molecular Toxicology-a Journal of Basic and Applied Research. ,vol. 13, pp. 145- 151 ,(2000) , 10.1089/109793300440730
Rosilla F. Bachmann, Robert J. Schloesser, Todd D. Gould, Husseini K. Manji, Mood Stabilizers Target Cellular Plasticity and Resilience Cascades: Implications for the Development of Novel Therapeutics Molecular Neurobiology. ,vol. 32, pp. 173- 202 ,(2005) , 10.1385/MN:32:2:173