Increase in intracellular free/bound NAD[P]H as a cause of Cd-induced oxidative stress in the HepG2 cells
作者： MS Yang , Dong Li , T Lin , JJ Zheng , Wei Zheng
DOI: 10.1016/J.TOX.2008.01.021
关键词:
摘要: The present study shows the use of confocal autofluorescence spectroscopy coupled with time-resolved fluorescence decay analysis to measure changes in FAD/NAD[P]H and free/bound NAD[P]H HepG2 cells at 0.5, 1.5, 3 4.5 h after exposure cadmium chloride (Cd). These were compared GSSG/GSH production reactive oxygen radicals (ROS) production. results demonstrated that both increased significantly upon Cd. change occurred as early 1.5 treatment while did not occur until exposure. Production ROS was also h. ratio studied. It 0.5 remained elevated provides novel data show metabolism precedes increase cellular oxidative stress (increase GSSG/GSH, FAD/NAD[P]H). is suggested Cd causes a release NAD[P]H, an important cofactor for electron transfer, from its normal protein binding sites. This may result disruption activity enzyme proteins, lead subsequent toxic events. © 2008 Elsevier Ireland Ltd. All rights reserved.
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