Gene expression profiles in human peripheral blood mononuclear cells as biomarkers for nutritional in vitro and in vivo investigations

作者: Thomas Hofmann , Stefanie Klenow , Anke Borowicki , Chris I. R. Gill , Beatrice L. Pool-Zobel

DOI: 10.1007/S12263-010-0170-1

关键词:

摘要: Identification of chemopreventive substances may be achieved by measuring biological endpoints in human cells vitro. Since generally only tumour are available for such investigations, our aim was to test the applicability peripheral blood mononuclear (PBMC) as an vitro primary cell model since they mimic vivo situation and relatively easily available. Cell culture conditions were refined, basal variation gene expression related drug metabolism stress response determined. Results compared with profiles established colon line (HT29) standard. For biomarker development nutritional effects, PBMC HT29 treated potentially (chrysin butyrate), Key results that relevant genes, glutathione S-transferase T2 (GSTT2) GSTM2, modulated butyrate cells, but less sensitive responded high individual differences. We conclude these serve a surrogate tissue dietary investigations identified differentially expressed genes have potential become marker population studies on effects.

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