Multilineage differentiation of human-derived dermal fibroblasts transfected with genes coated on PLGA nanoparticles plus growth factors.
作者： Ji Sun Park , Han Na Yang , Dae Gyun Woo , Su Yeon Jeon , Keun-Hong Park
DOI: 10.1016/J.BIOMATERIALS.2012.10.001
关键词:
摘要: Wounded tissues and cells may be treated with growth factors specific genes for the purpose of tissue repair regeneration. To deliver into cells, this study presents use fabricated poly (DL-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) complexed cationic polymer (ethleneimine) (PEI). Through complexation PEI, several types (SOX9, Cbfa1, C/EBP-α) were coated PLGA NPs, which enhanced gene uptake normal human-derived dermal fibroblast (NFDHCs) in vitro vivo. Several cell (293T, HeLa, cells) transfected fluorescence-tagged PEI/SOX9, PEI/Cbfa1, PEI/C/EBP-α gene-complexed NPs. The protein expression levels evaluated by RT-PCR, real-time quantitative PCR, Western blotting, confocal laser microscopy. Fibroblast encapsulated fibrin gels NPs plus (TGF-β3, BMP-2, or IGF/bFGF), induced chondrogenesis, osteogenesis, adipogenesis both after transplantation nude mouse.
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