Cationic glycopolymers for the delivery of pDNA to human dermal fibroblasts and rat mesenchymal stem cells.

摘要: Progenitor and pluripotent cell types offer promise as regenerative therapies but transfecting these sensitive cells has proven difficult. Herein, a series of linear trehalose-oligoethyleneamine "click" copolymers were synthesized examined for their ability to deliver plasmid DNA (pDNA) two progenitor types, human dermal fibroblasts (HDFn) rat mesenchymal stem (RMSC). Seven polymer vehicle analogs in which three parameters systematically varied: the number secondary amines (4-6) within repeat unit (Tr4(33), Tr5(30), Tr6(32)), end group functionalities [PEG (Tr4(128)PEG-a, Tr4(118)PEG-b), triphenyl (Tr4(107)-c), or azido (Tr4(99)-d)], molecular weight (degree polymerization about 30 100) biological efficacy vehicles was compared controls: Lipofectamine 2000, JetPEI, Glycofect. The trehalose polymers all able bind compact pDNA polyplexes, promote uptake gene expression [luciferase enhanced green fluorescent protein (EGFP)] with primary results varied significantly depending on structure. Interestingly, both Tr4(33) Tr5(30) yielded highest luciferase expression. However, when comparing transfected reporter encoding protein, Tr4(107)-c HDFn positive EGFP. RMSCs, higher Tr4(118)PEG-b, Tr4(107)-c, Tr4(99)-d) high percentages EGFP (30-40%).