Current state and future perspective of molecular diagnosis of fine-needle aspiration biopsy of thyroid nodules.

摘要: Context: Fine-needle aspiration biopsy (FNAB) is the most sensitive and specific tool for differential diagnosis of thyroid malignancy. Some limitations FNAB can be overcome by molecular analysis FNAB. This review analyzes current state problems as well possible goals increasing diagnostic rate, especially in indeterminate/follicular lesion cytological group. Evidence Acquisition: Twenty publications were evaluated material assay systems used, type, number mutations screened. Sensitivity, specificity, falsenegative false-positive rates calculated all publications. Synthesis: Testing a panel somatic promising to reduce indeterminate A mean sensitivity 63.7% was achieved lesions. However, there broad range investigation Therefore, additional markers should defined mRNA microRNAexpressionstudiesandevaluatedinFNABsamplesofthyroidcarcinomaswithoutknown mutations, many benign nodules fine-needle cytology category. approach improve samples. Conclusion: has led an improvement sensitivity/ specificity proliferation Further methodological improvements, standardizations, further soon lead broader application substantial reduction surgeries. (J Clin Endocrinol Metab 96: 2016–2026, 2011)