High frequency of ras oncogene activation in all stages of human thyroid tumorigenesis.

摘要: Using polymerase chain reaction amplification and oligonucleotide probing, the activation of ras oncogenes in 24 benign 20 malignant human thyroid neoplasms was examined. The frequency oncogene similar at all stages tumorigenesis this system, being found 33% adenomas overall (50% microfollicular tumours), 53% differentiated follicular carcinomas 60% undifferentiated carcinomas. This supports contention that mutation these occurs an early step tumorigenesis. predominant amino acid substitution tumours glutamine to arginine position 61 Ha-ras or N-ras, but not any tumours. It noted while transition mutations predominated (both malignant), transversions were more common