Downregulation of miR‑224‑5p in prostate cancer and its relevant molecular mechanism via TCGA, GEO database and in silico analyses.

摘要: The function of the expression microRNA (miR)-224-5p in prostate adenocarcinoma (PCa) remains to be elucidated, therefore, present study aimed investigate clinical significance and potential molecular mechanism miR-224-5p PCa. Data on PCa were extracted from Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), ArrayExpress previous literature, meta-analyses with standardized mean difference (SMD) summary receiver operating characteristic (sROC) methods performed for statistical analyses. prospective target genes collected by overlapping differentially expressed mRNAs TCGA GEO, predicted miRWalk2.0. Subsequently, silico analysis was examine associated pathways markedly lower PCa; overall SMD −0.562, sROC area under curve 0.80. In addition, Kyoto Encyclopedia Genes Genomes revealed that largely enriched amino sugar nucleotide metabolism signaling pathway, three [UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1), hexokinase 2 (HK2) chitinase (CHIT1)] this pathway showed higher (P<0.05). key protein-protein interaction network [DNA topoisomerase 2-α (TOP2A), ATP citrate lyase (ACLY) ribonucleotide reductase regulatory subunit M2 (RRM2)] exhibited significantly increased results suggested downregulated may progression prognosis Furthermore, likely exerts its effects targeting genes, including UAP1, HK2, CHIT1, TOP2A, ACLY RRM2. However, vivo vitro experiments are required confirm these findings.