Studying Amino Acid Transport Using Liposomes
作者： Cesare Indiveri
DOI: 10.1007/978-1-60761-447-0_5
关键词:
摘要: The transport of amino acid across the membranes has great importance in cell metabolism. Specific experimental methodologies are required for measuring vectorial reactions catalyzed by membrane transporters. So far, most widely used technique to study was measure flux intact systems expressing a specific transporter. Some limitations this procedure caused presence endogenous transporters and intracellular enzymes inaccessibility compartment. Alternative strategies which allow reducing interferences improving handling internal compartment would be useful knowledge.An protocol, makes use liposomes mediated glutamine/amino (ASCT2) transporter, solubilized from rat kidney brush borders, is described. based on reconstitution transporter removal detergent mixed micelles detergent, protein, phospholipid. assayed formed proteoliposomes Na(+) dependent uptake L: -[(3)H]glutamine antiport with -glutamine. This method allows activity under well controlled conditions permits performing experiments cannot realized systems.
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Bröer S, Bröer A, Lang F, Wagner Ca, Brookes N, Dimmer Ks, Ganapathy, The astroglial ASCT2 amino acid transporter as a mediator of glutamine efflux. Journal of Neurochemistry. ,vol. 73, pp. 2184- 2194 ,(1999)
Ferdinando Palmieri, Cesare Indiveri, Faustino Bisaccia, Vito Iacobazzi, Mitochondrial metabolite carrier proteins: purification, reconstitution, and transport studies. Methods in Enzymology. ,vol. 260, pp. 349- 369 ,(1995) , 10.1016/0076-6879(95)60150-3
John, D. McGivan, The transport of glutamine into mammalian cells Frontiers in Bioscience. ,vol. 12, pp. 874- 882 ,(2007) , 10.2741/2109
Naoko Utsunomiya-Tate, Hitoshi Endou, Yoshikatsu Kanai, Cloning and Functional Characterization of a System ASC-like Na+-dependent Neutral Amino Acid Transporter Journal of Biological Chemistry. ,vol. 271, pp. 14883- 14890 ,(1996) , 10.1074/JBC.271.25.14883
Reinhard Krämer, Claudia Heberger, Functional reconstitution of carrier proteins by removal of detergent with a hydrophobic ion exchange column. Biochimica et Biophysica Acta. ,vol. 863, pp. 289- 296 ,(1986) , 10.1016/0005-2736(86)90269-5
Yoshikatsu Kanai, Matthias A. Hediger, The glutamate/neutral amino acid transporter family SLC1: molecular, physiological and pharmacological aspects. Pflügers Archiv: European Journal of Physiology. ,vol. 447, pp. 469- 479 ,(2004) , 10.1007/S00424-003-1146-4
Bryan Mackenzie, Jeffrey D. Erickson, Sodium-coupled neutral amino acid (System N/A) transporters of the SLC38 gene family Pflügers Archiv: European Journal of Physiology. ,vol. 447, pp. 784- 795 ,(2004) , 10.1007/S00424-003-1117-9
Julie Lim, Karen A. Lorentzen, Joerg Kistler, Paul J. Donaldson, Molecular identification and characterisation of the glycine transporter (GLYT1) and the glutamine/glutamate transporter (ASCT2) in the rat lens Experimental Eye Research. ,vol. 83, pp. 447- 455 ,(2006) , 10.1016/J.EXER.2006.01.028
Lorena Pochini, Francesca Oppedisano, Cesare Indiveri, Reconstitution into liposomes and functional characterization of the carnitine transporter from renal cell plasma membrane Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1661, pp. 78- 86 ,(2004) , 10.1016/J.BBAMEM.2003.12.001
Francesca Oppedisano, Lorena Pochini, Michele Galluccio, Mariangela Cavarelli, Cesare Indiveri, Reconstitution into liposomes of the glutamine/amino acid transporter from renal cell plasma membrane: functional characterization, kinetics and activation by nucleotides Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1667, pp. 122- 131 ,(2004) , 10.1016/J.BBAMEM.2004.09.007