Proteoliposomes as Tool for Assaying Membrane Transporter Functions and Interactions with Xenobiotics

摘要: Proteoliposomes represent a suitable and up to date tool for studying membrane transporters which physiologically mediate absorption, excretion, trafficking reabsorption of nutrients metabolites. Using recently developed reconstitution strategies, can be inserted in artificial bilayers with the same orientation as cell membranes absence other interfering molecular systems. These methodologies are very kinetic parameters mechanisms. After first applications on mitochondrial transporters, last decade, proteoliposomes obtained optimized have been used plasma defining their functional properties structure/function relationships. A lot information has clarified completed knowledge several among OCTN sub-family members, neutral amino acid, B0AT1 ASCT2, others. Transporters absorption substrate-like derivatives or drugs, improving bioavailability interact these compounds xenobiotics, leading side/toxic effects. Therefore, interaction some toxic compounds, such mercurials, H2O2 drugs. Several mechanisms defined cases acid residues responsible identified. The data indicate novel potentially important drug discovery.