Role of Hematopoietic Growth Factors on the ex Vivo Expansion of Primitive Cord Blood Stem Cells

摘要: Ex vivo expansion of hematopoietic cells has recently been suggested for clinical application.1.2 It demonstrated that compartments derived from human bone marrow (BM), mobilized peripheral blood (PB) or umbilical cord (CB) can be maintained and expanded in liquid culture stroma coculture systems by the provision combinations growth factors.3–5 The potential benefits such studies include accelerated engraftment, reduced risk infection, smaller stem cell harvests, improved effectiveness genetically modified cells.1.2 Although controversies remain concerning what defined populations may not useful improving hematologic recovery, ex only considered successful when progeny receptor retain both pluripotent differentiation self-renewal capacities original cells.4 In other words, are various factor able to amplify late progenitor reservoir without exhausting pool, order ensure long-term post-transplantation engraftment after a myeloablative conditioning regimen? Human employing Interleukin 3 (IL3) Granulocyte-Colony Stimulating Factor (G-CSF) maintain at least some primitive cells;6–9 however, achieving net early proven elusive, although laboratories have reported encouraging results.10–12 With discovery new factors, specific cells, investigators re-examined possibility expanding vitro. particular, three cytokines discovered 1990’s, c-kit ligand Stem Cell (KL), flt3 (FL), c-mpl also termed Megakaryocyte Growth Development (MGDF) Thrombopoietin (TPO), appear unique activities on progenitor/stem cells.