Subcellular localization of marine bacterial alkaline phosphatases.

摘要: Bacterial alkaline phosphatases (APases) are important enzymes in organophosphate utilization the ocean. The subcellular localization of APases has significant ecological implications for marine biota but is largely unknown. extensive metagenomic sequence databases from Global Ocean Sampling Expedition provide an opportunity to address this question. A bioinformatics pipeline was developed identify bacterial databases, and a consensus classification algorithm designed predict their localizations. We identified 3,733 APase sequences (including PhoA, PhoD, PhoX) found that cytoplasmic (41%) extracellular (30%) exceed periplasmic (17%), outer membrane (12%), inner (0.9%) counterparts. unexpectedly high abundance suggests transport intracellular hydrolysis small molecules mechanism acquisition phosphorus (P) surface On average, each bacterium possessed at least one suite uptake glycerol phosphate (ugp) genes (e.g., ugpA, ugpB, ugpC, ugpE) dissolved organic (DOP) transport, only half them had ugpQ, which hydrolyzes transported DOP, indicating play role hydrolyzing DOP. most abundant heterotrophic bacteria, α- γ-Proteobacteria, might hydrolyze DOP outside membrane, former could also cytoplasm. bioavailable P organisms cannot directly access organophosphates, thereby increase biological productivity diversity.