Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii
作者： R.A.Y.S.A. Gunasekara , P. Cornillie , C. Casteleyn , W. De Spiegelaere , P. Sorgeloos
DOI: 10.1111/J.1365-2672.2010.04862.X
关键词:
摘要: Aims: Validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect Aeromonas hydrophila on gut development germ-free Artemia franciscana nauplii. Methods Results: Germ-free nauplii were cultured using Baker’s yeast dead Aer. hydrophila. Live Aer. hydrophila added first day to treatment group. The length volume monitored days two four reconstruction. Both methods showed comparable results. Stereology was least labour intensive estimate volumes, while reconstructions rendered architectural topographical data gut. Moreover, a positive bacterium, is likely. Conclusion: Slight increment in growth digestive tract A. franciscana exerted by bacteria could be detected reconstruction. Significance Impact Study: gnotobiotic rearing system unique investigate effects micro-organisms nauplii. However, base this model system, only survival counts measurements exist as tools. Therefore, additional tools such are prerequisite obtain more powerful analysis.
sci-hub.se 参考文章(31)
Gerald D. Abrams, Helmunz Sprinz, Heinz Bauer, INFLUENCE OF THE NORMAL FLORA ON MUCOSAL MORPHOLOGY AND CELLULAR RENEWAL IN THE ILEUM. A COMPARISON OF GERM-FREE AND CONVENTIONAL MICE Laboratory Investigation. ,vol. 12, pp. 355- 364 ,(1963)
J. F. Rawls, B. S. Samuel, J. I. Gordon, Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 4596- 4601 ,(2004) , 10.1073/PNAS.0400706101
R.A.Y.S. Asanka Gunasekara, Anamaria Rekecki, Kartik Baruah, Peter Bossier, Wim Van den Broeck, Evaluation of probiotic effect of Aeromonas hydrophila on the development of the digestive tract of germ-free Artemia franciscana nauplii Journal of Experimental Marine Biology and Ecology. ,vol. 393, pp. 78- 82 ,(2010) , 10.1016/J.JEMBE.2010.07.006
Bernhard Ruthensteiner, Martin Heß, Embedding 3D models of biological specimens in PDF publications. Microscopy Research and Technique. ,vol. 71, pp. 778- 786 ,(2008) , 10.1002/JEMT.20618
H. J. G. Gundersen, E. B. Jensen, The efficiency of systematic sampling in stereology and its prediction Journal of Microscopy. ,vol. 147, pp. 229- 263 ,(1987) , 10.1111/J.1365-2818.1987.TB02837.X
A. Rekecki, K. Dierckens, S. Laureau, N. Boon, P. Bossier, W. Van den Broeck, Effect of germ-free rearing environment on gut development of larval sea bass (Dicentrarchus labrax L.) Aquaculture. ,vol. 293, pp. 8- 15 ,(2009) , 10.1016/J.AQUACULTURE.2009.04.001
Pieter Cornillie, Wim Van Den Broeck, Paul Simoens, Three-dimensional reconstruction of the remodeling of the systemic vasculature in early pig embryos. Microscopy Research and Technique. ,vol. 71, pp. 105- 111 ,(2008) , 10.1002/JEMT.20531
Antonio Marques, Jean Dhont, Patrick Sorgeloos, Peter Bossier, Evaluation of different yeast cell wall mutants and microalgae strains as feed for gnotobiotically grown brine shrimp Artemia franciscana Journal of Experimental Marine Biology and Ecology. ,vol. 312, pp. 115- 136 ,(2004) , 10.1016/J.JEMBE.2004.06.008
Christophe Casteleyn, Wim Van den Broeck, Paul Simoens, Histological characteristics and stereological volume assessment of the ovine tonsils Veterinary Immunology and Immunopathology. ,vol. 120, pp. 124- 135 ,(2007) , 10.1016/J.VETIMM.2007.07.010
K.-A. Dorph-Petersen, J. R. Nyengaard, H. J. G. Gundersen, Tissue shrinkage and unbiased stereological estimation of particle number and size. Journal of Microscopy. ,vol. 204, pp. 232- 246 ,(2001) , 10.1046/J.1365-2818.2001.00958.X