Induction of the c-fos proto-oncogene in the rat pineal gland during stress

摘要: To investigate the effects of stressful stimuli on pineal gland activity, male Wistar albino rats (200-250 g, 2-4 per group) were submitted to 30 min forced immobilization or unilateral vibrissotomy before sacrifice. In situ hybridization was performed with a 35 S-labelled 50-base oligonucleotide probe complementary nucleotides 270-319 rat c-fos sections containing gland. Autoradiograms quantified using JAVA microdensitometer