Highly purified CD38+ sub-populations show no evidence of preferential clonal evolution despite having increased proliferative activity when compared with CD38- sub-populations derived from the same chronic lymphocytic leukaemia patient

摘要: In agreement with a recently published manuscript, this present study demonstrated that CD38 + sub-populations had increased proliferative activity as evidenced by higher Ki-67 expression (P < 0.0001). This raised the possibility fraction is exposed to an risk of clonal evolution. However, serial fluorescence in situ hybridisation analysis highly purified and - from individual patients revealed no distinct cytogenetic lesions or evidence preferential evolution fractions when compared their counterparts = 0.13). Furthermore, telomere length all similarly short telomeres 0.31) comparably low telomerase (TERT) 0.75) 0.88). Subsequent examination cell-sorted paired peripheral blood bone marrow samples taken on same day showed significant difference CD38, Ki-67, TERT lengths, indicating these chronic lymphocytic leukaemia cells were derived single pool trafficking between two compartments. Taken together, our data show bimodal have extensive histories undergone similar number cell divisions mirrored episodic CD38.