External Quality Assessment Unravels Interlaboratory Differences in Quality of RAS Testing for Anti-EGFR Therapy in Colorectal Cancer

作者: Véronique Tack , Marjolijn J.L. Ligtenberg , Lien Tembuyser , Nicola Normanno , Sara Vander Borght

DOI: 10.1634/THEONCOLOGIST.2014-0382

关键词:

摘要: BACKGROUND: Regulations for the selection of patients with metastatic colorectal cancer anti-EGFR treatment changed at end 2013. The set mutations to be tested extended from KRAS codons 12 and 13 NRAS exons 2, 3, 4. A European external quality assessment scheme monitored performance laboratories evaluated implementation new regulations. MATERIALS AND METHODS: 131 participating received 10 samples formalin-fixed paraffin-embedded material, including RAS (exon 4) BRAF mutations. Mock clinical data were provided three cases. Using their routine methods, determined genotypes submitted written reports. Assessors scored results according predefined evaluation criteria. RESULTS: Half participants (49.3%) had completely implemented test requirements (codons 12, 13, 59, 61, 117, 146 NRAS), 96 (73.3%) made no genotype mistakes. Correct nomenclature, Human Genome Variation Society, was used by 82 (62.6%). CONCLUSION: Although regulations effective several months, many not ready full testing in context therapy. Nevertheless, each country, there are that provide complete correct testing. External assessments can monitor stimulate improve procedures. Because this program available on website Society Pathology, clinicians refer a reliable laboratory.

参考文章(16)
Lien Tembuyser, Marjolijn J.L. Ligtenberg, Nicola Normanno, Sofie Delen, J. Han van Krieken, Elisabeth M.C. Dequeker, Higher Quality of Molecular Testing, an Unfulfilled Priority: Results from External Quality Assessment for KRAS Mutation Testing in Colorectal Cancer The Journal of Molecular Diagnostics. ,vol. 16, pp. 371- 377 ,(2014) , 10.1016/J.JMOLDX.2014.01.003
Elisabeth Dequeker, Marjolijn J. L. Ligtenberg, Sara Vander Borght, Johan H. J. M. van Krieken, Mutation analysis of KRAS prior to targeted therapy in colorectal cancer: development and evaluation of quality by a European external quality assessment scheme Virchows Archiv. ,vol. 459, pp. 155- 160 ,(2011) , 10.1007/S00428-011-1094-X
Chih-Chieh Chen, Tze-Kiong Er, Yen-Yi Liu, Jenn-Kang Hwang, Maria Jesus Barrio, Maximiliano Rodrigo, Enrique Garcia-Toro, Marta Herreros-Villanueva, None, Computational Analysis of KRAS Mutations: Implications for Different Effects on the KRAS p.G12D and p.G13D Mutations PLoS ONE. ,vol. 8, pp. e55793- ,(2013) , 10.1371/JOURNAL.PONE.0055793
V. Heinemann, J.Y. Douillard, M. Ducreux, M. Peeters, Targeted therapy in metastatic colorectal cancer -- an example of personalised medicine in action. Cancer Treatment Reviews. ,vol. 39, pp. 592- 601 ,(2013) , 10.1016/J.CTRV.2012.12.011
Jean-Yves Douillard, Kelly S Oliner, Salvatore Siena, Josep Tabernero, Ronald Burkes, Mario Barugel, Yves Humblet, Gyorgy Bodoky, David Cunningham, Jacek Jassem, Fernando Rivera, Ilona Kocákova, Paul Ruff, Maria Błasińska-Morawiec, Martin Šmakal, Jean Luc Canon, Mark Rother, Richard Williams, Alan Rong, Jeffrey Wiezorek, Roger Sidhu, Scott D Patterson, None, Panitumumab-FOLFOX4 treatment and RAS mutations in colorectal cancer The New England Journal of Medicine. ,vol. 369, pp. 1023- 1034 ,(2013) , 10.1056/NEJMOA1305275
Ellen Bellon, Marjolijn J.L. Ligtenberg, Sabine Tejpar, Karen Cox, Gert Hertogh, Karin Stricker, Anders Edsjö, Vassilis Gorgoulis, Gerald Höfler, Andreas Jung, Athanassios Kotsinas, Pierre Laurent‐Puig, Fernando López‐Ríos, Tine Plato Hansen, Etienne Rouleau, Peter Vandenberghe, Johan J.M. Krieken, Elisabeth Dequeker, External Quality Assessment for KRAS Testing Is Needed: Setup of a European Program and Report of the First Joined Regional Quality Assessment Rounds Oncologist. ,vol. 16, pp. 467- 478 ,(2011) , 10.1634/THEONCOLOGIST.2010-0429
Marc Peeters, Timothy Jay Price, Andrés Cervantes, Alberto F. Sobrero, Michel Ducreux, Yevhen Hotko, Thierry André, Emily Chan, Florian Lordick, Cornelis J.A. Punt, Andrew H. Strickland, Gregory Wilson, Tudor-Eliade Ciuleanu, Laslo Roman, Eric Van Cutsem, Valentina Tzekova, Simon Collins, Kelly S. Oliner, Alan Rong, Jennifer Gansert, Randomized Phase III Study of Panitumumab With Fluorouracil, Leucovorin, and Irinotecan (FOLFIRI) Compared With FOLFIRI Alone As Second-Line Treatment in Patients With Metastatic Colorectal Cancer Journal of Clinical Oncology. ,vol. 28, pp. 4706- 4713 ,(2010) , 10.1200/JCO.2009.27.6055