Growth and analysis of crystal forms of toxic shock syndrome toxin 1.

作者: Cathleen A. Earhart , G. Sridhar Prasad , Debra L. Murray , Richard P. Novick , Patrick M. Schlievert

DOI: 10.1002/PROT.340170310

关键词:

摘要: Native toxic shock syndrom toxin 1 (TSST-1) purified from Staphylococcus aurius has been crystallized in four different forms. The highest resolution data (2.05 A) was collected orthorhombic crystals belonging to the space group C2221. unit cell dimension are a = 108.7 A, b 177.5 c 97.6 A. Rotation function analysis of this indicates that there is trimer molecules asymmetric with local 3-fold axis parallel crystallographic axis. Crystals double mutant TSST-1 have grown which single molecule and diffract 1.9 P21 parameters 44.4 34.0 55.2 β 93.0°. © 1993 Wiley-Liss, Inc.

参考文章(21)
Debra A Blomster-Hautamaa, Patrick M Schlievert, Preparation of toxic shock syndrome toxin-1. Methods in Enzymology. ,vol. 165, pp. 37- 43 ,(1988) , 10.1016/S0076-6879(88)65009-9
R.E. Hunt, K. Moffat, D.W. Golde, Purification and crystallization of the polypeptide hormone human chorionic somatomammotropin. Journal of Biological Chemistry. ,vol. 256, pp. 7042- 7045 ,(1981) , 10.1016/S0021-9258(19)69096-4
D A Blomster-Hautamaa, B N Kreiswirth, J S Kornblum, R P Novick, P M Schlievert, The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. Journal of Biological Chemistry. ,vol. 261, pp. 15783- 15786 ,(1986) , 10.1016/S0021-9258(18)66787-0
Gregory A. Bohach, Young-In Chi, Cynthia V. Stauffacher, Crystallization and preliminary X-ray diffraction analysis of staphylococcal enterotoxin type C. Proteins. ,vol. 13, pp. 152- 157 ,(1992) , 10.1002/PROT.340130208
M. G. Rossmann, D. M. Blow, The detection of sub‐units within the crystallographic asymmetric unit Acta Crystallographica. ,vol. 15, pp. 24- 31 ,(1962) , 10.1107/S0365110X62000067
S. Swaminathan, Daniel S.C. Yang, William Furey, Leatrice Abrams, James Pletcher, Martin Sax, Crystallization and preliminary X-ray study of staphylococcal enterotoxin B. Journal of Molecular Biology. ,vol. 199, pp. 397- ,(1988) , 10.1016/0022-2836(88)90326-9
X Paliard, S. West, J. Lafferty, Clements, J. Kappler, P Marrack, B. Kotzin, Evidence for the effects of a superantigen in rheumatoid arthritis Science. ,vol. 253, pp. 325- 329 ,(1991) , 10.1126/SCIENCE.1857971
L Imberti, A Sottini, A Bettinardi, M Puoti, D Primi, Selective depletion in HIV infection of T cells that bear specific T cell receptor V beta sequences. Science. ,vol. 254, pp. 860- 862 ,(1991) , 10.1126/SCIENCE.1948066