A method for screening the temperature dependence of three-dimensional crystal formation
作者： Michael J. Landsberg , James Bond , Christine L. Gee , Jennifer L. Martin , Ben Hankamer
DOI: 10.1107/S0907444906006093
关键词:
摘要: Temperature is an important parameter controlling protein crystal growth. A new temperature-screening system (Thermo-screen) described consisting of a gradient thermocycler fitted with special crystallization-plate adapter onto which 192-well sitting-drop crystallization plate can be mounted (temperature range 277–372 K; maximum temperature 20 K; interval precision 0.3 K). The allows 16 different conditions to monitored simultaneously over 12 temperatures and well suited conduct wide (∼20 K) fine (∼3 K) temperature-optimization screens. It potentially aid in the determination phase diagrams run more complex temperature-cycling experiments for seeding
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