Serological Specificity of the Lipopolysaccharides, the Major Antigens of Pseudomonas syringae
作者： Christians Guillorit , Regine Samson
DOI: 10.1111/J.1439-0434.1993.TB01335.X
关键词:
摘要: The nature of major antigens Pseudomonas syringae was studied on one strain four pathovars (pvs aptata, mors-prunorum, phaseolicola and tabaci) belonging to separate serogroups. Bacterial were prepared by 4 procedures: extraction phenol-water (PW), citrate-NaCl (CN), trichloracetic acid (TCA), precipitation a glycoproteic extracellular complex (GP). 3-Deoxy-2-octulosonic (KDO) revelation in all the extracts showed that procedures led containing similar amounts lipopolysaccharide (LPS). Twenty polyclonal antisera raised rabbits against whole bacteria different extracts. Serological reactions tested gel double diffusion (DD) indirect immunofluorescent staining (IF). The anti-whole cell sera shown contain mostly anti-LPS antibodies. For each pathovar, used as DD gave bands identical given LPS (PW, CN or TCA), heated (HB), sidechain preparations. GP extract itself be rich LPS. To serotype P. syringae, it is advised raise either extracts; whereas reacting for would bacteria.
sci-hub.se 参考文章(24)
J E Cadieux, J Kuzio, F H Milazzo, A M Kropinski, Spontaneous release of lipopolysaccharide by Pseudomonas aeruginosa. Journal of Bacteriology. ,vol. 155, pp. 817- 825 ,(1983) , 10.1128/JB.155.2.817-825.1983
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Timothy C. RAY, Anthony R. W. SMITH, Robin WAIT, Richard C. HIGNETT, Structure of the sidechain of lipopolysaccharide from Erwinia amylovora T. FEBS Journal. ,vol. 170, pp. 357- 361 ,(1987) , 10.1111/J.1432-1033.1987.TB13707.X
Yashwant D. Karkhanis, Johanna Y. Zeltner, Jesse J. Jackson, Dennis J. Carlo, A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteria Analytical Biochemistry. ,vol. 85, pp. 595- 601 ,(1978) , 10.1016/0003-2697(78)90260-9
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
N. Thaveechai, Comparison of Different Immunogen Preparations for Serological Identification ofXanthomonas campestrispv.campestris Phytopathology. ,vol. 74, pp. 1065- 1070 ,(1984) , 10.1094/PHYTO-74-1065
S.A. Barker, Susan M. Bick, J.S. Brimacombe, P.J. Somers, Purification of pneumococcus types II and V polysaccharides Carbohydrate Research. ,vol. 1, pp. 393- 399 ,(1966) , 10.1016/S0008-6215(00)81468-8
J. D. Otta, Serology and Pathology of Pseudomonas syringae Phytopathology. ,vol. 61, pp. 443- ,(1971) , 10.1094/PHYTO-61-443
Anthony R. W. SMITH, Susanne E. ZAMZE, Susan M. MUNRO, Kathleen J. CARTER, Richard C. HIGNETT, Structure of the sidechain of lipopolysaccharide from Pseudomonas syringae pv. morsprunorum C28 FEBS Journal. ,vol. 149, pp. 73- 78 ,(1985) , 10.1111/J.1432-1033.1985.TB08895.X
Hacene Bouzar, Importance of Ribosome Purity in Ribosomal Serology Phytopathology. ,vol. 76, pp. 1323- 1325 ,(1986) , 10.1094/PHYTO-76-1323