Purification of pneumococcus types II and V polysaccharides
作者： S.A. Barker , Susan M. Bick , J.S. Brimacombe , P.J. Somers
DOI: 10.1016/S0008-6215(00)81468-8
关键词: Polysaccharide 、 Alcohol 、 Fractionation 、 Sephadex 、 Biochemistry 、 Nucleic acid 、 Chemistry 、 Complex formation 、 Chromatography 、 DEAE Sephadex
摘要: Abstract DEAE Sephadex has been shown to afford separation of Type V Pneumococcus specific polysaccharide, C-substance, and other components, when alcohol fractionation or detergent precipitation were only partially effective. Dissociable complex formation between II polysaccharide nucleic acid demonstrated on the same column. G-200 can be used remove impurities from polysaccharide.
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