Matrix metalloproteinase-9, a potential biological marker in invasive pituitary adenomas

摘要: Object We analyzed MMP-9 expression using mRNA and protein level determinations explored the possibility that Matrix metalloproteinase-9 (MMP-9) is a potential biological marker of pituitary adenoma invasiveness whether could be used to discriminate extent among different hormonal subtypes, tumor sizes, growth characteristics, primary versus recurrent tumors. Materials methods 73 specimens were snap frozen in liquid nitrogen immediately after surgical resection. RNA extracted. transcripts by quantitative RT-PCR. activity was gelatin zymography validated western blot analysis. Immunohistochemistry performed identify presence localization adenomas. Statistical differences between results determined Student’s t-test or one way ANOVA. Results Comparing subtypes noninvasive invasive tumors, significantly increased majority Considering levels, our data also showed significant increase adenomas these confirmed analysis immunohistochemistry. In addition, consistent levels found according subtype, size, extension redo-surgery. Conclusions can consistently distinguish tumors from would reflect extension, redo surgery, even at early stages invasiveness. may considered biomarker determine predict nature