Typing of Campylobacter pylori by bacterial DNA restriction endonuclease analysis and determination of plasmid profile.

摘要: Campylobacter pylori isolates from 37 symptomatic patients and 3 asymptomatic volunteers were examined by chromosomal DNA restriction endonuclease analysis determination of plasmid profile. Restriction digests with HindIII, HaeIII, PvuII, BglII produced clear reproducible results that permitted discrimination between different strains. Only 35% C. found to have DNA. Isolates patients, including those two pairs siblings, had unique patterns profiles. Consecutive obtained 1 year apart each identical profiles, suggesting persistence the same strain. A pair one third volunteer differed in content but similar patterns. Plasmid profile bacterial provide a reliable means discriminating strains may be useful for typing these organisms epidemiologic studies.