Characterization and presumptive identification of Helicobacter pylori isolates from rhesus monkeys.
作者： E S Drazek , A Dubois , R K Holmes
DOI: 10.1128/JCM.32.7.1799-1804.1994
关键词:
摘要: We characterized 38 Helicobacter isolates, including 22 from gastric biopsy samples obtained 14 rhesus monkeys and single isolates 16 in a different colony. Biochemical profiles of these were nearly identical to that pylori ATCC 43504. Restriction fragment length polymorphism (RFLP) analysis indicated each infected monkey harbored one four strains. The 17 RFLP types found among differed all seven RFLPs the other isolates. Thus, within given colony are more likely be by with same or similar than colonies. A 16S rRNA gene was amplified PCR cloned isolate 85D08. Ribotyping this probe demonstrated less diversity reported H. humans, as did ureA-ureB cluster. DNA sequence determined compared sequences for species. Our 127 nucleotides (corresponding residues 1240 1366 Escherichia coli gene) 85D08 99.2 100% homologous humans but only 83.5 96.9% species region gene. These data provide strong support presumptive identification pylori.
sci-hub.st 参考文章(26)
W. P. C. Stemmer, A 20-minute ethidium bromide/high-salt extraction protocol for plasmid DNA. BioTechniques. ,vol. 10, pp. 726- ,(1991)
Andre Dubois, Nancy Fiala, Lillie M. Heman-Ackah, E.Susan Drazek, Andrzej Tarnawski, William N. Fishbein, Guillermo I. Perez-Perez, Martin J. Blaser, Natural gastric infection with Helicobacter pylori in monkeys: a model for spiral bacteria infection in humans. Gastroenterology. ,vol. 106, pp. 1405- 1417 ,(1994) , 10.1016/0016-5085(94)90392-1
A R Euler, G E Zurenko, J B Moe, R G Ulrich, Y Yagi, Evaluation of two monkey species (Macaca mulatta and Macaca fascicularis) as possible models for human Helicobacter pylori disease. Journal of Clinical Microbiology. ,vol. 28, pp. 2285- 2290 ,(1990) , 10.1128/JCM.28.10.2285-2290.1990
P A Foxall, L T Hu, H L Mobley, Use of polymerase chain reaction-amplified Helicobacter pylori urease structural genes for differentiation of isolates. Journal of Clinical Microbiology. ,vol. 30, pp. 739- 741 ,(1992) , 10.1128/JCM.30.3.739-741.1992
Stuart L. Hazell, Jorg W. Eichberg, D.Rick Lee, Leslie Alpert, Dolores G. Evans, Doyle J. Evans, David Y. Graham, Selection of the chimpanzee over the baboon as a model for Helicobacter pylori infection. Gastroenterology. ,vol. 103, pp. 848- 854 ,(1992) , 10.1016/0016-5085(92)90016-R
S A Ho, J A Hoyle, F A Lewis, A D Secker, D Cross, N P Mapstone, M F Dixon, J I Wyatt, D S Tompkins, G R Taylor, Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals. Journal of Clinical Microbiology. ,vol. 29, pp. 2543- 2549 ,(1991) , 10.1128/JCM.29.11.2543-2549.1991
C A McNulty, J C Dent, Rapid identification of Campylobacter pylori (C. pyloridis) by preformed enzymes. Journal of Clinical Microbiology. ,vol. 25, pp. 1683- 1686 ,(1987) , 10.1128/JCM.25.9.1683-1686.1987
P J Romaniuk, B Zoltowska, T J Trust, D J Lane, G J Olsen, N R Pace, D A Stahl, Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp. Journal of Bacteriology. ,vol. 169, pp. 2137- 2141 ,(1987) , 10.1128/JB.169.5.2137-2141.1987
A E Simor, B Shames, B Drumm, P Sherman, D E Low, J L Penner, Typing of Campylobacter pylori by bacterial DNA restriction endonuclease analysis and determination of plasmid profile. Journal of Clinical Microbiology. ,vol. 28, pp. 83- 86 ,(1990) , 10.1128/JCM.28.1.83-86.1990
C. S. Goodwin, S. I. H. Majewski, Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation The Journal of Infectious Diseases. ,vol. 157, pp. 465- 471 ,(1988) , 10.1093/INFDIS/157.3.465