PCR detection of colonization by Helicobacter pylori in conventional, euthymic mice based on the 16S ribosomal gene sequence.

摘要: Many animal models of Helicobacter infection have been described, including in rhesus monkeys, ferrets, gnotobiotic piglets, and mice. These utilize a combination detection methods, culture, urease testing, histopathology, all which may be unreliable, insensitive, or labor-intensive. Development new pylori requires methods with increased sensitivity specificity. We developed sensitive specific PCR primers based on the 16S ribosomal gene sequence H. pylori. The detected single-copy DNA representing 0.2 cell pure (2 cells presence mouse stomach mucosal DNA) did not cross-react closely related bacteria. were able to detect colonization by conventional, euthymic, outbred mice up 4 weeks postinoculation high percentage isolates tested. One isolate was 100% at 6 months 60% 1 year after inoculation. Approximately 10(3) 10(4) per utilizing this methodology semiquantitatively. facilitated euthymic mice, detectable other methods.