Carboxylic acids permeases in yeast: two genes in Kluyveromyces lactis.

摘要: Two new genes KlJEN1 and KlJEN2 were identified in Kluyveromyces lactis. The deduced structure of their products is typical membrane-bound carriers displays high similarity to Jen1p, the monocarboxylate permease Saccharomyces cerevisiae. Both are under control glucose repression mediated by FOG1 FOG2, corresponding S. cerevisiae GAL83 SNF1 respectively, KlCAT8, proteins involved signalling cascade K. KlJEN1, but not KlJEN2, induced lactate. contrast expressed at level ethanol succinate. physiological characterization null mutants showed that functional homologue ScJEN1, whereas encodes a dicarboxylic acids transporter. In fact, KlJen1p [transporter classification (TC) number: 2.A.1.12.2.] required for lactate uptake therefore growth on KlJen2p succinate transport, as demonstrated experiments inability Kljen2 mutant grow This carrier appears transport also malate fumarate because cannot these substrates competed carboxylic acids. We conclude first yeast gene shown encode permease.