B-type allatostatin modulates immune response in hepatopancreas of the mud crab Scylla paramamosain

摘要: B-type allatostatin (AST-B) is a pleiotropic neuropeptide, widely found in arthropods. However, the information about its immune effect crustaceans unknown. In this study, we identified nervous tissue as main site for Sp-AST-B expression, while receptor gene (Sp-AST-BR) expressed various tissues, including hepatopancreas. This suggests peptide's potential role diverse physiological processes mud crab Scylla paramamosain. situ hybridization revealed that Sp-AST-BR mainly localized F-cell of Furthermore, significant up-regulation transcripts hepatopancreas following exposure to lipopolysaccharide (LPS) or polyriboinosinic polyribocytidylic acid (Poly (I:C)). Results from vitro and vivo experiments treatment with synthetic AST-B peptide mediated upregulation expression AST-BR, nuclear factor-κB (NF-κB) pathway components (Dorsal Relish), pro-inflammatory cytokine (IL-16) antimicrobial peptides (AMPs) addition, elevation nitric oxide (NO) production enhanced bacteriostasis capacity vitro. Taken together, these findings reveal existence basic neuroendocrine-immune (NEI) network crabs, indicate could couple trigger downstream signaling pathways induce responses