Novel siRNA formulation to effectively knockdown mutant p53 in osteosarcoma

摘要: Objectives The tumor suppressor p53 plays a crucial role in the development of osteosarcoma. The primary objective this study is to develop and optimize lipid based nanoparticle formulations that can carry siRNA effectively silence mutant 318–1, murine osteosarcoma cell line. Methods nanoparticles were composed mixture two lipids (cholesterol DOTAP) either PLGA or PLGA-PEG prepared by using an EmulsiFlex-B3 high pressure homogenizer. A series studies include different nanoparticles, amount siRNAs, numbers, incubation time, transfection media volume, storage temperature was performed gene silencing efficiency. Key findings Replacement decreased particle size overall cytotoxicity. Among all lipid-polymer nanoformulations, with 10% showed highest knockdown efficiency while maintaining higher viability when ratio equal 6.8:0.66 75 nM used. With long term greater extent. Conclusions This warrants future evaluation formulation for tissue culture animal models treatment