Repetitive sequences in complete and defective genomes of Herpesvirus saimiri.

摘要: Two types of Herpesvirus saimiri genomes can be isolated from purified virions: (i) the M genome is a double-stranded, liniear DNA molecule with mean contour length corresponding to 89 times 10-6 daltons. The contains about 70% unique sequences (light DNA, 36% guanine plus cytosine) and 30% reiterated (heavy 71% cytosine). (ii) H composed heavy only more heterogeneous in size. are up 40-fold reiterated, indicating defectiveness this type genome. repetitions cross-hybridize almost completely have identical kinetic complexity (2.8 daltons). infectivity studies by using calcium phosphate DEAE-dextran method gave further evidence that defective: no infectious virus was recovered permissive cells treated whereas genome-infected developed cytopathic changes after 11 56 days. Defective were present progeny two passages transfection.