Consumption of vitamin B6 reduces colonic damage and protein expression of HSP70 and HO-1, the anti-tumor targets, in rats exposed to 1,2-dimethylhydrazine
作者： TOMOKO KAYASHIMA , KENTA TANAKA , YUKAKO OKAZAKI , KIMINORI MATSUBARA , NORIYUKI YANAKA
DOI: 10.3892/OL.2011.370
关键词:
摘要: Mounting evidence indicates that vitamin B6 is a protective factor for colon cancer. Elevations in colonic damage, cell proliferation and heat shock proteins (HSPs, molecular chaperones) have been suggested to be associated with carcinogenesis. This study was performed examine the effect of dietary levels (1, 7 or 35 mg pyridoxine HCl/kg diet) 22 weeks on epithelial expression HSPs rats exposed 1,2-dimethylhydrazine (DMH). Supplemental low diet (1 significantly reduced fecal activity intestinal alkaline phosphatase (an index damage) epithelium PCNA labeling (a marker proliferation). Analysis using ELISA indicated supplemental lowered protein HSP70 heme oxygenase-1, HSP32 (HO-1). However, real-time RT-PCR analysis revealed mRNA these were not decreased by B6, suggesting lowering mediated mechanisms involving altered gene expression. provided suppresses HO-1, targets anti-tumor agents, DMH.
sci-hub.se 参考文章(26)
Alexzander Asea, Stine-Kathrein Kraeft, Evelyn A. Kurt-Jones, Mary Ann Stevenson, Lan Bo Chen, Robert W. Finberg, Gloria C. Koo, Stuart K. Calderwood, HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine. Nature Medicine. ,vol. 6, pp. 435- 442 ,(2000) , 10.1038/74697
Noriyuki Yanaka, Shun-Ichiro Komatsu, Masami Isobe, Norihisa Kato, A high-fat diet enhances the inhibitory effect of dietary vitamin B6 on colon cell proliferation in mice. Oncology Reports. ,vol. 14, pp. 265- 269 ,(2005) , 10.3892/OR.14.1.265
John A. Lapré, Hielke T. de Vries, Roelof van der Meer, Cytotoxicity of fecal water is dependent on the type of dietary fat and is reduced by supplemental calcium phosphate in rats. Journal of Nutrition. ,vol. 123, pp. 578- 585 ,(1993) , 10.1093/JN/123.3.587
Susanna C. Larsson, Nicola Orsini, Alicja Wolk, Vitamin B6and Risk of Colorectal Cancer JAMA. ,vol. 303, pp. 1077- 1083 ,(2010) , 10.1001/JAMA.2010.263
Philip G. Reeves, Forrest H. Nielsen, George C. Fahey, AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet Journal of Nutrition. ,vol. 123, pp. 1939- 1951 ,(1993) , 10.1093/JN/123.11.1939
Shun-ichiro KOMATSU, Hiromitsu WATANABE, Tatsuzo OKA, Haruhito TSUGE, Norihisa KATO, Dietary Vitamin B6 Suppresses Colon Tumorigenesis, 8-Hydroxyguanosine, 4-Hydroxynonenal, and Inducible Nitric Oxide Synthase Protein in Azoxymethane-Treated Mice Journal of Nutritional Science and Vitaminology. ,vol. 48, pp. 65- 68 ,(2002) , 10.3177/JNSV.48.65
Noriyuki Yanaka, Taka-Aki Koyama, Shun-Ichiro Komatsu, Eri Nakamura, Mayumi Kanda, Norihisa Kato, Vitamin B6 suppresses NF-κB activation in LPS-stimulated mouse macrophages International Journal of Molecular Medicine. ,vol. 16, pp. 1071- 1075 ,(2005) , 10.3892/IJMM.16.6.1071
Claudio R. Teixeira, Shinji Tanaka, Ken Haruma, Masaharu Yoshihara, Koji Sumii, Goro Kajiyama, Proliferating cell nuclear antigen expression at the invasive tumor margin predicts malignant potential of colorectal carcinomas. Cancer. ,vol. 73, pp. 575- 579 ,(1994) , 10.1002/1097-0142(19940201)73:3<575::AID-CNCR2820730313>3.0.CO;2-0
Pamela Kanellis, Mark Gagliardi, Judit P Banath, Rachel K Szilard, Shinichiro Nakada, Sarah Galicia, Frederic D Sweeney, Diane C Cabelof, Peggy L Olive, Daniel Durocher, A Screen for Suppressors of Gross Chromosomal Rearrangements Identifies a Conserved Role for PLP in Preventing DNA Lesions PLOS Genetics. ,vol. 3, ,(2007) , 10.1371/JOURNAL.PGEN.0030134
Kaeko Hirai, Tomonori Sasahira, Hitoshi Ohmori, Kiyomu Fujii, Hiroki Kuniyasu, Inhibition of heme oxygenase‐1 by zinc protoporphyrin IX reduces tumor growth of LL/2 lung cancer in C57BL mice International Journal of Cancer. ,vol. 120, pp. 500- 505 ,(2007) , 10.1002/IJC.22287