Inhibition of the activity of DNA polymerase α by 2′,3′-dideoxythymidine 5′-triphosphate
作者： Katsuhiko Ono , Masako Ogasawara , Akio Matsukage
DOI: 10.1016/0006-291X(79)91115-X
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摘要: Abstract 2′,3′-Dideoxythymidine 5′-triphosphate was found to strongly inhibit the activity of DNA polymerase α from mouse myeloma in presence manganese ion as divalent cation. The extent inhibition by 2′,3′-dideoxythymidine increased raising pH reaction. mode competitive substrate, 2′-deoxythymidine 5′-triphosphate. Ki for (0.035 μM) much lower than Km (1.8 μM).
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