Urokinase-type plasminogen activator receptor regulates apoptotic sensitivity of colon cancer HCT116 cell line to TRAIL via JNK-p53 pathway
作者： Kai Wang , Ping-Kun Zhou , Yao Wang , Zi-Chun Hua , Xiufeng Liu
DOI: 10.1007/S10495-014-1025-9
关键词:
摘要: The urokinase-type plasminogen activator receptor (uPAR) serves not only as an anchor for but also participates in intracellular signal transduction events. In this study, we investigated whether uPAR could modulate TRAIL-induced apoptosis human colon cancer cells HCT116. Using antisense strategy, established a stable HCT116 cell line with down-regulated uPAR. sensitivity to was evaluated by FACS analysis. Our results show that the inhibition of sensitize apoptosis. changed expression mitochondrial apoptotic pathway proteins, including Bcl-2, Bax, Bid and p53, pro-apoptotic manner. We found phosphorylation FAK, ERK JNK. p53 RNA interference rescued from enhanced apoptosis, thus indicating is critical enhancing Furthermore, JNK, ERK, involved up-regulation p53. JNK negatively regulated protein level. Overall, our can via active pathways involved.
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