Stability of antibody-conjugated gold nanoparticles in the endolysosomal nanoenvironment: implications for noninvasive radiofrequency-based cancer therapy

摘要: Abstract The use of noninvasive radiofrequency (RF) electric fields as an energy source for thermal activation nanoparticles within cancer cells could be a valuable addition to the emerging field nano-mediated therapies. Based on investigations cell death through hyperthermia, and offering ability total-body penetration by RF fields, this technique is thought complement possibly outperform existing nano-heat treatments that utilize alternative heat production via optical or magnetic stimuli. However, it remains challenge understand fully complex RF-nanoparticle-intracellular interactions before full system optimization can engineered. Herein we have shown liver selectively internalize antibody-conjugated gold (AuNPs) receptor-mediated endocytosis, with predominantly accumulating aggregating cytoplasmic endolysosomes. After exposure external field, nonaggregated AuNPs absorbed dissipated heat, causing damage targeted cells. We also observed absorption dissipation dependent solubility in colloid, which pH dependent. Furthermore, modulating endolysosomal possible prevent intracellular AuNP aggregation enhance cytotoxicity hepatocellular From Clinical Editor Gold absorb from exert hyperthermic effects leading death. Combining known effect antibody-based targeting nanoparticles, selective specific hyperthermia induced therapies designed, demonstrated article.