Structural characterization of the mouse long-chain acyl-CoA dehydrogenase gene and 5' regulatory region.

摘要: Long-chain acyl-CoA dehydrogenase (LCAD) is one of four enzymes involved in the initial step mitochondrial β-oxidation straight-chain fatty acids. It a member (Acad or ACAD) gene family enzymes, which also includes very-long-chain (VLCAD), medium-chain (MCAD), and short-chain (SCAD) dehydrogenases. These all have similar activity but differ only chain length specificity for their substrate. Mitochondrial provides an important source energy especially during times fasting. In order to understand role LCAD this pathway, we cloned characterized entire mouse (Mus muscu-lus) encoding (Acadl). Acadl single-copy, nuclear encoded approximately 35 kb size. We sequenced coding region, intron/exon boundaries, 1.7 its 5′ regulatory mapped transcription start site. The contains 11 exons ranging size from 67 bp 275 bp, interrupted by 10 introns 1.0 6.6 like other members Acad family, lacks TATA CAAT box GC rich. This region does contain multiple, putative cis-acting DNA elements recognized either SP1 steroid-thyroid receptors, has been shown with ACAD be regulated expression. characterization will allow further study vivo model, how expression may coordinated family.