Membrane protein solubilization.

摘要: A critical step in any vitro analysis of membrane proteins is the solubilization to extract protein interest an active form obtain aqueous solution containing complexed with detergents and lipids a suitable for purification further analysis. This process particularly delicate as aim maximally disrupt lipid components while putting un-natural detergent environment without perturbing them. Looked at this way, it remarkable that ever works. Although difficult hard master, increasing number have been successfully solubilized forms, allowing some general principles be established we illustrate method developed chapter.