Alkylsilyl speciation and direct sample preparation of plant cannabinoids prior to their analysis by GC-MS

摘要: Abstract A literature criticism is given on methods currently using gas chromatography mass spectrometry (GC-MS) to determine plant cannabinoids (p-CBDs). In this study, trialkylsilylation of seven p-CBDs (including their transformation products formed in the drug user's body) was compared applying various alkylsilyl reagents 1 and fragmentation properties corresponding derivatives were characterized. Derivatization, quantitation related model investigations optimized as a function reaction times conditions. Special emphasis put (i) maximum responses species, (ii) proportions stable products, suitable for selective all simultaneously. Results, novel field confirmed that HMDS + TFA, never applied reagent before, serves derivatization choice. These species characterized by retention, analytical performance characteristics. solutions with injected amounts range 20 pg–2000 pg, repeatability (average 4.98% RSD, varying between 2.98 6.2% RSD), linearity (R2, 0.9956–0.9995), LOQ (20–80 pg/μL species) recovery (95.2–104%) values defined. The practical utility proposal, along method development validation, shown particularly unique manner supported novel, extraction free, direct sample preparation working strategy. For purpose, two Cannabis-type ruderalis (C-trd) tissues (C-trd1, C-trd2) directly derivatized presence matrix. This process, which approaches green chemistry, performed without use organic solvents, associated self p-CBD contents C-trd tissues. Applying 0.5–2.0 mg dried tissues, adding standards, following confirmed: C-trd1 6.6 μg/mg CBD, 4.4 μg/mg CBN 1.3 μg/mg CBC, while C-trd2 0.46 μg/mg 0.27 μg/mg CBC 0.19 μg/mg CBG found. latter results (2.52–4.99% 0.9640–0.9997) (87.9–109%) data.