Detection of cannabigerol and its presumptive metabolite in human urine after Cannabis consumption.

摘要: Kemp et al. (1995) could detect delta9-tetrahydrocannabinol (delta9-THC), cannabinol and cannabidiol, three neutral cannabinoids, the metabolites of delta9-THC in urine samples Cannabis consumers. In this study we aimed to identify cannabigerol (CBG), which its acid form is one main intermediate compounds biosynthesis cannabinoids hemp, authority proved For reason applied modified method test for CBG, since enzymatic hydrolysis seems be necessary formation free from conjugates. After extraction, derivatisation with N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) GC/MS analysis, peaks characteristic fragment ions (m/z 337, 391, 377 460) bis-trimethylsilyl derivative CBG appeared at 12.48 minutes both real sample spiked CBG. It shows that enters body during smoking excreted a conjugated form, like other cannabinoids. Analysing chromatograms hydrolysed trimethylsilylated extracts checked presence CBG-metabolites based on Harvey Brown (1990). We detected compound consumers' extracts, having m/z 425, 465 479 retention time 14.19 min presumed 4"-hydroxy-CBG or 5"-hydroxy-CBG. However, it not identified completely by GC/MS. This peak was absent non-hydrolysed samples, indicating also glucuronated form.