Detection of Parasitic Plant Suicide Germination Compounds Using a High-Throughput Arabidopsis HTL/KAI2 Strigolactone Perception System

摘要: Strigolactones are terpenoid-based plant hormones that act as communication signals within a plant, between plants and fungi, parasitic their hosts. Here we show an active enantiomer form of the strigolactone GR24, germination stimulant karrikin, number structurally related small molecules called cotylimides all bind HTL/KAI2 α/β hydrolase in Arabidopsis. also promoted interaction F-box protein MAX2 yeast. Identification this chemically dependent protein-protein prompted development yeast-based, high-throughput chemical screen for potential mimics. Of 40 lead compounds identified, three were found to have planta activity using Arabidopsis-based assays. More importantly, these stimulate suicide obligate Striga hermonthica. These results suggest screening strategies involving yeast/Arabidopsis models may be useful combating infestations.