Identification of putative novel class-I lanthipeptides in firmicutes : A combinatorial in silico analysis approach performed on genome sequenced bacteria and a close inspection of Z-geobacillin lanthipeptide biosynthesis gene cluster of the Thermophilic geobacillus sp. strain ZGt-1

摘要: Lanthipeptides are ribosomally synthesized and post-translationally modified polycyclic peptides. that have antimicrobial activity known as lantibiotics. Accordingly, the discovery of novel lantibiotics constitutes a possible solution for problem antibiotic resistance. We utilized publicly available genome sequences bioinformatic tools tailored detection lanthipeptides. designed our strategy screening 252 firmicute genomes detecting class-I lanthipeptide-coding gene clusters. The resulted in identifying 69 lanthipeptide sequences, which more than 10% were putative novel. identified lanthipeptides not been annotated on original or RefSeq genomes, merely coding hypothetical proteins. Additionally, we bacterial strains previously recognized lanthipeptide-producers. Moreover, suggest corrections certain annotations, recommend records enriching bacteriocin mining tool (BAGEL) databases. Furthermore, propose Z-geobacillin, coded thermophilic strain Geobacillus sp. ZGt-1. provide lists unrecognized lanthipeptide-producing strains, so they can be prioritized experimental investigation. Our results expected to benefit researchers interested vitro production