Membrane surface charge dictates the structure and function of the epithelial Na+/H+ exchanger.
作者： Robert Todd Alexander , Valentin Jaumouillé , Tony Yeung , Wendy Furuya , Iskra Peltekova
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摘要: The Na+/H+ exchanger NHE3 plays a central role in intravascular volume and acid–base homeostasis. Ion exchange activity is conferred by its transmembrane domain, while regulation of the rate transport variety stimuli dependent on cytosolic C-terminal region. Liposome- cell-based assays employing synthetic or recombinant segments tail demonstrated preferential association with anionic membranes, which was abrogated perturbations that interfere electrostatic interactions. Resonance energy transfer measurements indicated domain approach bilayer. In intact cells, neutralization basic residues mutagenesis disruption interactions inhibited activity. An switch model proposed to account for multiple aspects
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