作者: Asen Daskalov , Patrick S. Mitchell , Andrew Sandstrom , Russell E. Vance , N. Louise Glass
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摘要: … Beads were washed six times in lysis buffer and then denatured in 2× SDS loading buffer. Samples were separated on NuPAGE Bis-Tris 4–12% gradient gels (ThermoFisher) following …