Highly sensitive amperometric immunosensors for microcystin detection in algae
作者： Mònica Campàs , Jean-Louis Marty
DOI: 10.1016/J.BIOS.2006.04.025
关键词:
摘要: Abstract The presence of cyanobacterial toxins in water and algae pose a health hazard for animals humans, due to their tumour-promoting activity carcinogen effects. use simple, rapid reliable tools routine analysis is becoming necessity. With this purpose, our group has developed two electrochemical immunosensors the detection microcystin-LR (MC-LR) based on affinity between cyanotoxin corresponding monoclonal polyclonal antibodies. A competitive direct enzyme-linked immunosorbent assays (ELISAs) was designed and, after validation approach microtiter wells, screen-printed graphite electrodes were used as supports. Colorimetry optimise experimental parameters compare performance Afterwards, measurements performed at −200 mV (versus Ag/AgCl) using 5-methyl-phenazinium methyl sulfate (MPMS) mediator horseradish peroxidase (HRP), enzymatic label competitor. IC 50 values 0.10 1.73 μg L −1 MAb PAb, respectively. Whereas Mab provided higher sensitivities, reproducibility better when PAb. amperometric applied samples from Tarn River (Midi-Pyrenees, France) MC confirmed by colorimetric protein phosphatase inhibition (PPI) assay high liquid chromatography (HPLC). limits attained calibration curves results obtained real demonstrate potential screening assessment quality control algae.
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