Chemotactic response of splenic mononuclear cells to angiotensin II in murine schistosomiasis.

摘要: Murine Schistosomiasis mansoni is a parasitic infection associated with delayed-type hypersensitivity granulomatous reaction to the schistosome eggs. This characterized by accumulation of mononuclear cells and other inflammatory cell types around Granuloma macrophages produce angiotensin II (AII), which appears have immunoregulatory function. By using an in vitro chemotaxis assay, this study demonstrated that AII chemotactic factor for splenic derived from infected mice. The response was bimodal, peak activities occurring at 10(-10) 10(-6) M. T lymphocytes, B large population unidentified optimal concentrations peptide. At high concentrations, also phagocytic cells. Sar1, ala8-AII, analog antagonist activity, completely blocked AII-induced chemotaxis. A [3H]-AII binding assay revealed high-affinity specific on spleen rapid, dependent radioligand concentration, reversible. These latter observations suggest activity subpopulations mediated via receptors.