Heterologous expression of Trichoderma atroviride endochitinase ech42 in Pichia pastoris at low and high dissolved oxygen tensions
作者： A. De León-Rodríguez , A. P. Barba de la Rosa , A.S. Pérez-Martínez , A.S. Pérez-Martínez
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摘要: La expresion de la Endoquitinasa 42 kDa Trichoderma atroviride recombinante (rech42) en Pichia pastoris bajo direccion del promotor constitutivo, PGAP, fue analizada a baja (3%) y alta (40%) tension oxigeno disuelto (TOD). Las actividades enzimaticas volumetricas maximas logradas cada condicion fueron 18,600±1053 12,330±1000 U 1−1, TOD 3% 40%, respectivamente. Mientras que las especificas 89.4±4.6 88.8±4.0 mg−1proteina. Se encontro conjuncion una sobreexpresion rech42 afecto el tamano celulas pero no actividad enzimatica especifica
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