Effect of PCB 126 and PCB 153 on incidence of apoptosis in cultured theca and granulosa cells collected from small, medium and large preovulatory follicles.

摘要: The aim of the presented study was to evaluate effects PCB 126 and 153 on granulosa theca cell apoptosis. Granulosa cells were collected from small, medium, large preovulatory porcine follicles cultured as monolayers. Cells initially for 24 h allow attachment plates. Media changed 100 pg/ml or ng/ml added. After 48 h, fixed assessment number apoptotic utilizing a Hoechst staining technique frozen measurement caspase-3 activity. testosterone concentration analysis cultures estradiol cultures. Neither nor had an effect secretion by small medium size follicles, while both PCBs decreased follicles. decrease in under influence paralleled suppression activity incidence bodies. increased PCB-associated accompanied In conclusion, we have evidence that inhibit Therefore, exposure may cause alterations pattern terminal differentiation attenuate spontaneous elimination atretic