Epigenetic conversion of adult dog skin fibroblasts into insulin-secreting cells.

摘要: Diabetes is among the most frequently diagnosed endocrine disorder in dogs and its prevalence continues to increase. Medical management of this pathology lifelong challenging because numerous serious complications. A therapy based on use autologous viable insulin-producing cells replace lost β cell mass would be very advantageous. protocol enable epigenetic conversion canine dermal fibroblasts, obtained from a skin biopsy, into (EpiCC) described present manuscript. Cells were briefly exposed DNA methyltransferase inhibitor 5-azacytidine (5-aza-CR) order increase their plasticity. This was followed by three-step differentiation that directed towards pancreatic lineage. After 36 days, 38 ± 6.1% treated fibroblasts converted EpiCC expressed insulin mRNA protein. Furthermore, able release medium response an increased glucose concentration. first evidence generating renewable autologous, functional source insulin-secreting possible dog. procedure represents novel promising potential for diabetes dogs.