Cellulose microfibril alignment recovers from DCB‐induced disruption despite microtubule disorganization

摘要: Cellulose microfibril deposition patterns define the direction of plant cell expansion. To better understand how alignment is controlled, we examined orientation during cortical microtubule disruption using temperature-sensitive mutant Arabidopsis thaliana, mor1-1. In a previous study, it was shown that at restrictive temperature for mor1-1, microtubules lose transverse and cells growth anisotropy without any change in parallel arrangement cellulose microfibrils. this investigated whether pre-existing template well-ordered microfibrils or presence well-organized essential to resume We first transiently disrupted order mor1-1 brief treatment with synthesis inhibitor 2,6-dichlorobenzonitrile (DCB). then analysed recently deposited (by field emission scanning electron microscopy) as recovered remained mutant's non-permissive culture temperature. Despite disordered an initially randomized wall texture, new were parallel, orientation. These results show requires neither accurately nor also demonstrated DCB treatments reduced ability form arrays, supporting role influencing organization.