Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions
作者： Shan Tang , Zhengpeng Wan , Yiren Gao , Ji-Shen Zheng , Jing Wang
DOI: 10.1039/C5SC03404C
关键词:
摘要: We report the chemical synthesis of first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells. This strategy facilitated fine tuning caged optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K96NPE, was totally inert hen egg lysozyme (HEL)-specific cells could only restore antigenicity upon photoactivation. Combined with real time live cell imaging, utility HEL-K96NPE demonstrated as a proof concept quantify synapse formation calcium influx at single level.
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