Evaluation of candidate reference genes for qPCR in maize.

作者: Alessandro Manoli , Alba Sturaro , Sara Trevisan , Silvia Quaggiotti , Alberto Nonis

DOI: 10.1016/J.JPLPH.2012.01.019

关键词:

摘要: Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels. Accurate and reproducible results are dependent on the correct choice of the reference …

参考文章(65)
Steve Rozen, Helen Skaletsky, Primer3 on the WWW for general users and for biologist programmers. Methods of Molecular Biology. ,vol. 132, pp. 365- 386 ,(2000) , 10.1385/1-59259-192-2:365
Amy M Brunner, Igor A Yakovlev, Steven H Strauss, Validating internal controls for quantitative plant gene expression studies BMC Plant Biology. ,vol. 4, pp. 14- 14 ,(2004) , 10.1186/1471-2229-4-14
Stephen A Bustin, Tania Nolan, Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. Journal of biomolecular techniques. ,vol. 15, pp. 155- 166 ,(2004)
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Paula Fernandez, Julio A. Di Rienzo, Sebastián Moschen, Guillermo A. A. Dosio, Luis A. N. Aguirrezábal, H. Esteban Hopp, Norma Paniego, Ruth A. Heinz, Comparison of predictive methods and biological validation for qPCR reference genes in sunflower leaf senescence transcript analysis. Plant Cell Reports. ,vol. 30, pp. 63- 74 ,(2011) , 10.1007/S00299-010-0944-3
Fernanda Cruz, Samara Kalaoun, Paula Nobile, Carlos Colombo, Juliana Almeida, Leila MG Barros, Eduardo Romano, Maria Fátima Grossi-de-Sá, Maité Vaslin, Marcio Alves-Ferreira, None, Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR Molecular Breeding. ,vol. 23, pp. 607- 616 ,(2009) , 10.1007/S11032-009-9259-X
Mukesh Jain, Aashima Nijhawan, Akhilesh K. Tyagi, Jitendra P. Khurana, Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR Biochemical and Biophysical Research Communications. ,vol. 345, pp. 646- 651 ,(2006) , 10.1016/J.BBRC.2006.04.140
Zhaoguo Tong, Zhihong Gao, Fei Wang, Jun Zhou, Zhen Zhang, Selection of reliable reference genes for gene expression studies in peach using real-time PCR BMC Molecular Biology. ,vol. 10, pp. 71- 71 ,(2009) , 10.1186/1471-2199-10-71
Hayati M. Iskandar, Robert S. Simpson, Rosanne E. Casu, Graham D. Bonnett, Donald J. Maclean, John M. Manners, Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane Plant Molecular Biology Reporter. ,vol. 22, pp. 325- 337 ,(2004) , 10.1007/BF02772676
Tony Remans, Karen Smeets, Kelly Opdenakker, Dennis Mathijsen, Jaco Vangronsveld, Ann Cuypers, Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations Planta. ,vol. 227, pp. 1343- 1349 ,(2008) , 10.1007/S00425-008-0706-4