Comprehensive Selection of Reference Genes for Gene Expression Normalization in Sugarcane by Real Time Quantitative RT-PCR

摘要: The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). In order to facilitate studies in sugarcane (Saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate genes was evaluated. geNorm, NormFinder and deltaCt methods were selecting stably expressed internal controls across different tissues under various experimental treatments. These results revealed that, among these genes, GAPDH, eEF-1a eIF-4α most stable suitable use factors all samples. addition, APRT could be examining relationship between copy number transcript levels tissue According evaluated by combining CUL eEF-1α hormone treatment experiments; CAC abiotic stress tests; samples plus CAC, CUL, TIPS-41 cultivar groups would lead more accurate reliable quantification sugarcane. This is first systematic validation profiles study should provide useful information other species.