Preservation of filamentous cyanobacteria cultures (Pseudanabaena galeata) Böcher and Geitlerinema amphibium (Ag. ex Gom.) Anagn. under low temperatures

摘要: Abstract A method for freezing preservation of the filamentous cyanobacteria Pseudanabaena galeata Bocher and Geitlerinema amphibium (Ag. ex Gom.) Anagn. at −30°C −80°C was examined. Four procedures, two different cryoprotectors growth states were assayed. The use 5% v/v dimethyl sulphoxide as cryoprotectant produced better results than methanol 1.5 M. In general, recoveries over all storage temperatures procedures tested higher in G. P. , which more sensitive to intercellular trichome breakage. cultures stationary logarithmic phase respectively, with or −80°C, proved be a suitable conservation technique these cyanobacteria.